منابع مشابه
Nascent Ribosomes
The conversion of a ribosomal RNA transcript to a cytoplasmic ribosome requires hundreds of accessory RNA and protein factors. Two papers published recently in Molecular Cell provide first looks at the association of these processing factors with the intermediates in ribosome synthesis (Harnpicharnchai et al., 2001; Bassler et al., 2001).
متن کاملViewing folding of nascent polypeptide chains from ribosomes.
Regulation of gene expression occurs at multiple stages and ultimately determines the protein levels in cells. The last decade has witnessed impressive progress in the development of approaches to measuring changes in cellular transcription and protein complement. However, techniques monitoring protein synthesis have lagged far behind. Current proteomic studies gauge the steady state levels of ...
متن کاملEvidence for RNA linked to nascent DNA in HeLa cells
Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled component revealed that its sedimentation coefficient is in the range of 4S and that it exists in a single- and double-stranded conformation. Moreover, analysis by nitrocellulose chromatography and CsSO4 density sedimentation of the nascent ...
متن کاملPicky nascent peptides do not talk to foreign ribosomes.
O ne of the least understood aspects of protein synthesis is communication between the ribosome and the nascent peptide. Chiba et al. (1) in this issue of PNAS reveal that, at least in some cases, these dialogues can be species-specific. The ribosome assembles amino acids into proteins in the peptidyl transferase center (PTC) located at the interface side of the large ribosomal subunit. On its ...
متن کاملThe conformation of nascent polylysine and polyphenylalanine peptides on ribosomes.
Polypeptide synthesis using either phenylalanine or lysine was initiated on Escherichia coli ribosomes; then the position and conformation of the nascent peptide were monitored by fluorescence techniques. To this end, fluorophores had been attached to the amino terminus of each nascent peptide, and major differences were observed as chain extension occurred. Polyphenylalanine appeared to build ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1967
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.58.5.1984